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| Product | Description | |
|---|---|---|
| Agarose Molecular Biology Grade | Environmentally friendly recommended for Southern and Northern blotting applications where high purity and low sulphate is required. The gel also exhibits low background fluorescence after ethidium bromide staining. Categories: Laboratory Molecular & Microbiology Reagents, Agarose. Brand Fisher Biotec. SKU FBAG100. ID 4957 |  Medical & Lab Supplies |
| ELECTROPHORESIS KITS | From teachers discussions on electrophoresis at SCICON 2006 established that students need only to appreciate the principles and practice of electrophoresis. It is not necessary to obtain cut DNA samples to satisfactorily demonstrate the process. When using cut samples there is A requirement for A chemical, ethidium bromide, to highlight the DNA. This is A banned substance for use in schools - (Refer to the Ministry of Education manual Safety and Science revised edition page 57.) DNA Electrophoresis is routinely used as A means of separating fragments of DNA that differ in size. Samples are placed into wells at one end of an agarose gel, this is covered with A conducting buffer and an electrical current is passed across it. Because of its associated phosphate groups DNA has A net negative charge and will therefore naturally begin to migrate towards the anode. The agarose gel with its dense structure acts like A sieve, small fragments move easily through it but larger fragments, hindered by the gel, make slower progress. It is this differential speed of DNA pieces that cause them to separate out from each other. The dyes in the kit also migrate at variable speeds and can therefore be used to mimic the behaviour of different sized DNA fragments. The dyes that travel the greatest distance represent small pieces of DNA. The sample that make little progress are representative of larger pieces. The four dyes in the kit represent the following types of DNA: .. Suppliers to schools & laboratories of science equipment & laboratory grade chemicals. | .jpg) Science & Lab Supplies NZ |
| Flat Tip pH Electrode for Skin and Surfaces with BNC Connector - HI1413B | The HI1413B is a glass body, single reference junction pH electrode made with low temperature glass and a BNC connector. This electrode has a unique open junction design in which there is a viscolene (hard gel) electrolyte layer that is free of silver chloride (AgCl) between the sample to be measured and the internal reference cell. The open junction design resists clogging and the probe's flat tip makes it ideal for pH measurements on surfaces such as skin, leather, agar plates, and paper. Categories: Laboratory The Hanna Range, pH Meters and Electrodes, pH Electrodes. Brand Hanna Instruments. SKU HA-HI1413B. ID 28021 | Medical & Lab Supplies |
| Guide-it Genotype Confirmation Kit | Every cell has two alleles of most genes. CRISPR/Cas9 gene editing can introduce insertion or deletion mutations (indels) on either one or both alleles of a given gene. The Guide-it Genotype Confirmation Kit provides a simple method to determine if a given clone has mutations in one allele (monoallelic), both alleles (biallelic), or is unchanged (wild type). The protocol involves amplification of the target site from cells treated with Cas9 and a single guide RNA (sgRNA). The resulting amplicon is used in an in vitro cleavage reaction with recombinant Cas9 and the same sgRNA. The genotype can be determined by resolving the cleavage products on an agarose gel. Categories: Laboratory Molecular & Microbiology Reagents, Gene Editing. Brand Takara Bio. SKU CLT632611. ID 27057 | Medical & Lab Supplies |
| Guide-it Mutation Detection Kit | The Guide-it Mutation Detection Kit contains all the reagents needed for PCR-based identification of insertions or deletions generated in the genome of cells treated with engineered nucleases such as zinc-finger nucleases (ZFNs), transcription-activator-like effector nucleases (TALENs), or CRISPR/Cas9. The first step is the amplification of the putative target sequence directly from cells. This kit uses Terra PCR Direct Polymerase Mix and Buffer, so there is no need to extract genomic DNA from your cell population prior to amplification of your target sequence. The amplicon is then melted and hybridized to form the mismatched targets for cleavage by the Guide-it Resolvase. Agarose gel electrophoresis can be used to visualize the cleavage products. Categories: Laboratory Molecular & Microbiology Reagents, Gene Editing. Brand Takara Bio. SKU CLT631443. ID 24931 | Medical & Lab Supplies |
| Pterocladia Seaweed | Pterocladia Seaweed suppliers in NZ of wild harvest Agar. Categories: Pterocladia Seaweed Lucida Agar, East Coast Kelp, Sodium Alginate, Seaweed Products, Pterocladia lucida (Gelidiales) |  Wairarapa |
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