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| Product | Description | |
|---|---|---|
| BAC-Optimized Replicator v2.0 Electrocompetent Cells | BAC-Optimized Replicator v2.0 Electrocompetent Cells (> 1 1010 cfu/µg pKanR DNA). These cells are required for on-demand, copy number amplification of the pSMART BAC or pEZ BAC vectors in the CopyRight Kits, thereby increasing DNA yields. Categories: Laboratory Molecular & Microbiology Reagents, Competent Cells and Transfection Reagents. Brand Lucigen. SKU LUC-60210-1. ID 26949 |  Medical & Lab Supplies |
| BigEasy®-TSA Electrocompetent Cells | BigEasy -TSA Electrocompetent Cells (>2 1010 cfu/µg pKanR DNA) are for use with Lucigen's pJAZZ -OC or OK Linear Vectors in the BigEasy v2.0 Linear Cloning System. The TSA Cells have the telN gene required for high efficiency transformation, and the antA gene required for copy number control with the pJAZZ vector, integrated into the host genome. These cells also give high yield and high quality plasmid DNA due to the endA1 and recA1 mutations. They contain the mcr and mrr mutations, allowing methylated genomic DNA that has been isolated directly from mammalian or plant cells to be cloned without deletions or rearrangements. Categories: Laboratory Molecular & Microbiology Reagents, Competent Cells and Transfection Reagents. Brand Lucigen. SKU LUC-60224-2. ID 26959 | Medical & Lab Supplies |
| CJ236 Electrocompetent Cells | Lucigen is the sole provider of high-efficiency electrocompetent CJ236 cells. This cell line was created by adding the pCJ105 plasmid to BW313 cells2, and is ideally suited to the Kunkel directed mutagenesis method. This method relies on the cells producing plasmid DNA containing some uracil residues. The dut- and ung- mutations prevent the cells from removing uracil from DNA caused by misincorporation or dCTP deamination. Note that these cells contain an F' which confers resistance to Chloramphenicol at low levels. As Electrocompetent cells, the transformation efficiency is 1 109 cfu/µg. Categories: Laboratory Molecular & Microbiology Reagents, Competent Cells and Transfection Reagents. Brand Lucigen. SKU LUC-60701-1. ID 27027 | Medical & Lab Supplies |
| ClearColi® BL21(DE3) Electrocompetent Cells | ClearColi BL21(DE3) cells are the first commercially available competent cells with a modified LPS that does not trigger the endotoxic response in mammalian cells. ClearColi cells lack outer membrane agonists for hTLR4/MD-2 activation; therefore, activation of hTLR4/MD-2 signalling by ClearColi is several orders of magnitude lower compared with E. coli wild-type cells, and heterologous proteins prepared from ClearColi are virtually free of endotoxic activity. After minimal purification from ClearColi cells, proteins or plasmids, which may still contain Lipid IVA, can still be used in most applications without eliciting an endotoxic response. Categories: Laboratory Molecular & Microbiology Reagents, Competent Cells and Transfection Reagents. Brand Lucigen. SKU LUC-60810-1. ID 27039 | Medical & Lab Supplies |
| ClearColi® K-12 Electrocompetent Cells | ClearColi cells are the first commercially available competent cells with a modified LPS (Lipid IVA - see Fig. 1) that does not trigger the endotoxic response in mammalian cells. ClearColi cells lack outer membrane agonists for hTLR4/MD-2 activation; therefore, activation of hTLR4/MD-2 signalling by ClearColi is several orders of magnitude lower compared with E. coli wild-type cells, and plasmid DNA prepared from ClearColi is virtually free of endotoxic activity. Categories: Laboratory Molecular & Microbiology Reagents, Competent Cells and Transfection Reagents. Brand Lucigen. SKU LUC-60850-1. ID 27043 | Medical & Lab Supplies |
| E. cloni® 10G BAC-Optimized Electrocompetent Cells | E. cloni 10G BAC-Optimized Electrocompetent Cells (> 1 107 cfu/µg 150 kb BAC DNA; > 1 1010 cfu/µg pUC DNA). The first competent cells developed by Lucigen specifically for large DNA cloning. Manufactured using a novel procedure that maximizes transformation efficiencies with very large inserts, 10G BAC-Optimized Cells are an excellent choice for BAC library construction and large construct transformation. Categories: Laboratory Molecular & Microbiology Reagents, Competent Cells and Transfection Reagents. Brand Lucigen. SKU LUC-60215-1. ID 26953 | Medical & Lab Supplies |
| E. cloni® 10G Chemically Competent Cells | E. cloni 10G Competent Cells - routine cloning, sub cloning, and plasmid isolation with or without blue/white screening. Lucigen's E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5a , DH10B , JM109, TOP10, etc. and directly replace them in cloning protocols. E. cloni chemically competent cells provide the performance researchers need with ease of use - only common laboratory equipment is required. These cells provide solutions for a wide range of applications at economical prices. Categories: Laboratory Molecular & Microbiology Reagents, Competent Cells and Transfection Reagents. Brand Lucigen. SKU LUC-60096-1. ID 26925 | Medical & Lab Supplies |
| E. cloni® 10G Electrocompetent Cells | E. cloni 10G Competent Cells. Library construction, cloning, sub cloning, and plasmid isolation with or without blue/white screening. Lucigen's E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5a , DH10B , JM109, TOP10, etc. and directly replace them in cloning protocols. However, E. cloni electrocompetent cells incorporate a unique manufacturing technology that increases transformation efficiency, recombinant yields and reliability, while decreasing costs. These cells provide solutions for a wide range of applications at economical prices. Categories: Laboratory Molecular & Microbiology Reagents, Competent Cells and Transfection Reagents. Brand Lucigen. SKU LUC-60117-2. ID 26947 | Medical & Lab Supplies |
| E. cloni® 10GF' ELITE Electrocompetent Cells | E. cloni 10GF?Competent Cells. Contain the F' plasmid for infection with M13 to produce ssDNA. Lucigen's E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5a , DH10B , JM109, TOP10, etc. and directly replace them in cloning protocols. However, E. cloni electrocompetent cells incorporate a unique manufacturing technology that increases transformation efficiency, recombinant yields and reliability (Figures 1 and 2), while decreasing costs. These cells provide solutions for a wide range of applications at economical prices. Categories: Laboratory Molecular & Microbiology Reagents, Competent Cells and Transfection Reagents. Brand Lucigen. SKU LUC-60061-1. ID 26909 | Medical & Lab Supplies |
| E. cloni® 5-alpha Chemically Competent Cells | Lucigen's E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5a and directly replace them in cloning protocols. E. cloni chemically competent cells provide the performance researchers need with ease of use - only common laboratory equipment is required. These cells provide solutions for a wide range of applications at economical prices. Categories: Laboratory Molecular & Microbiology Reagents, Competent Cells and Transfection Reagents. Brand Lucigen. SKU LUC-60602-1. ID 27023 | Medical & Lab Supplies |
| E. cloni® EXPRESS BL21(DE3) Competent Cells | Lucigen's E. cloni EXPRESS BL21(DE3) Electrocompetent Cells are the first to offer high efficiency cloning and high level protein expression in the same cell. Cloning efficiencies are increased 25-1,000 fold relative to competitors' preparations of BL21 cells, which is essential for construction of complex expression libraries. The unprecedented transformation efficiency of E. cloni EXPRESS Electrocompetent Cells (> 5 109 cfu/µg) eliminates the need for plasmid transfer from the cloning strain to the expression strain, saving days of work in a typical cloning and expression experiment. Categories: Laboratory Molecular & Microbiology Reagents, Competent Cells and Transfection Reagents. Brand Lucigen. SKU LUC-60401-1. ID 26983 | Medical & Lab Supplies |
| Endura Competent Cells | Lucigen's Endura Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains. Clones with inverted repeats or other sequences prone to recombination are commonly found in retroviral genes, and require cells such as Endura to be propagated stably. Categories: Laboratory Molecular & Microbiology Reagents, Competent Cells and Transfection Reagents. Brand Lucigen. SKU LUC-60240-1. ID 26963 | Medical & Lab Supplies |
| HI-Control Chemically Competent Cells | The HI-Control strains are based on E. cloni 10G ( DH10B) and BL21(DE3) strains. These strains harbor a plasmid bearing a constitutive lacI repressor gene, which provides tight control over promoters containing the lacO operator. Categories: Laboratory Molecular & Microbiology Reagents, Competent Cells and Transfection Reagents. Brand Lucigen. SKU LUC-60110-1. ID 15923 | Medical & Lab Supplies |
| In-Fusion® HD Cloning Plus System | In-Fusion PCR Cloning Kits enable directional, seamless cloning of any PCR fragment - or multiple fragments - into any linearized vector in a single 15-minute reaction. No additional treatment of the PCR fragment is required (such as restriction digestion, ligation, phosphorylation, or blunt-end polishing). The efficiency of In-Fusion HD cloning kits is over 95%. Categories: Laboratory Molecular & Microbiology Reagents, Competent Cells and Transfection Reagents. Brand Takara Bio. SKU CLT638909. ID 15933 | Medical & Lab Supplies |
| OverExpress C41(DE3) and C43(DE3) Competent Cells | Lucigen's OverExpress Electrocompetent and Chemically Competent Cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins has been validated in more than 350 publications. Categories: Laboratory Molecular & Microbiology Reagents, Competent Cells and Transfection Reagents. Brand Lucigen. SKU LUC-60341-1. ID 26991 | Medical & Lab Supplies |
| Phage Display Competent Cells | Cell Lines available: ER2738 Electrocompetent Cells, SS320 (MC1061 F') Electrocompetent Cells, TG1 Electrocompetent Cells, MC1061 F- Electrocompetent Cells. Categories: Laboratory Molecular & Microbiology Reagents, Competent Cells and Transfection Reagents. Brand Lucigen. SKU LUC-60522-1. ID 27019 | Medical & Lab Supplies |
| Recovery Medium, 8 x 12 ml | Recovery Medium is provided as one box containing eight 12 mL vials of recovery medium. Recovery Medium should be stored at -20°C until just before use. For research use only. Not for human or diagnostic use. Categories: Laboratory Molecular & Microbiology Reagents, Competent Cells and Transfection Reagents. Brand Lucigen. SKU LUC-80026-1. ID 15883 | Medical & Lab Supplies |
| Xfect RNA Transfection Reagent | Xfect RNA Transfection Reagent is a complete system for highly efficient transfection of mammalian cells with all types of RNA. Transfection with messenger RNA allows for rapid gene expression, without the risk of genomic integration that comes from transfection with DNA. While most transfection reagents can be harmful to your cells, the Xfect RNA Transfection Reagent has very low cytotoxicity. The reagent can also efficiently deliver single guide RNAs (sgRNA) for CRISPR/Cas9-mediated gene editing. This reagent creates biodegradable nanoparticles when combined with RNA, enabling efficient transfection of mammalian cells without any of the damaging side-effects. Categories: Laboratory Molecular & Microbiology Reagents, Competent Cells and Transfection Reagents. Brand Takara Bio. SKU CLT631450. ID 27059 | Medical & Lab Supplies |
| Yeastmaker Carrier DNA | The Yeastmaker Yeast Transformation System 2 provides a high-efficiency polyethylene glycol (PEG)/LiAc-based method for preparing and transforming competent yeast cells. Size: 5 x 1 mL. Categories: Laboratory Molecular & Microbiology Reagents, Competent Cells and Transfection Reagents. Brand Takara Bio. SKU CLT630440. ID 75235 | Medical & Lab Supplies |
| Yeastmaker Yeast Transformation System 2 | The Yeastmaker Yeast Transformation System 2 provides a high-efficiency polyethylene glycol (PEG)/LiAc-based method for preparing and transforming competent yeast cells. Categories: Laboratory Molecular & Microbiology Reagents, Competent Cells and Transfection Reagents. Brand Takara Bio. SKU CLT630439. ID 26749 | Medical & Lab Supplies |
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