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Transposomics provides a fast and easy method for generating a library of DNA sequences with insertional mutations, or a library of live mutant bacteria. Many genomics applications bene t from the high e efficiency and low bias of the EZ-Tn5 system, including metagenomics, strain development, functional genomics and large fragment sequencing. Transposomics is easier than chemical mutagenesis, and is a trusted and well-established method for generating mutations across over 60 species of bacteria. With 100's of publications and a wide array of applications and tools available, you are limited only by your imagination. Categories: Laboratory Molecular & Microbiology Reagents. Brand Lucigen. SKU LUC-TSM99K2. ID 37513
Medical & Lab Supplies
AxyPrep PCR Cleanup Kit (250 preps)
The AxyPrep PCR Cleanup Kit employs a special Binding Solution in combination with an AxyPrep spin column to achieve high selectivity and recovery of DNA fragments. This product is designed to purify DNA fragments > 75 bp from PCRs and other enzymatic reactions, with an expected recovery of 70-90%. It is not necessary to remove mineral oil overlays from the PCRs before purification. This protocol will remove unincorporated primers (<50 not), enzymes and unlabelled and fluorescent dye-labelled mononucleotides. The purified DNA fragments are suitable for a variety of applications, such as sequencing, ligation, restriction analysis, in vitro transcription, etc. Categories: Laboratory Molecular & Microbiology Reagents, Nucleic Acid Extraction Clean Up Kits. Brand Corning Life Sciences. SKU AXY-AP-PCR-250. ID 13564
Medical & Lab Supplies
Culture Plates
Square-well Block with 2.1 mL square wells used for growing, harvesting, or lysing of bacterial cultures, with Gas-permeable Foil. Categories: Laboratory Molecular & Microbiology Reagents, Nucleic Acid Extraction Plasmid DNA Kits Molecular Biology Sequencing Grade Plasmid DNA. Brand Macherey-Nagel. SKU MN740488.24. ID 23815
Medical & Lab Supplies
dNTP Equimolar Mix, 10 mM in 1mL
Ready-to-use dNTP mixes are designed to save researchers time and minimize the possibility of contamination because of the fewer pipetting steps required. They are supplied as an aliquot of an equimolar mix of each nucleotide (dATP, dCTP, dGTP, dTTP). They are stable for 12 months when stored at -20oC and ideally suited for use with DNA polymerases in PCR and in the labelling and sequencing of DNA. Categories: Laboratory Molecular & Microbiology Reagents, PCR Reagents Dntps. Brand Fisher Biotec. SKU FBDN-10M. ID 9263
Medical & Lab Supplies
EpiXplore Meth-Seq DNA Enrichment Kit
The EpiXplore Meth-Seq DNA Enrichment Kit is a complete system for separating methylated and unmethylated fractions of sheared genomic DNA, and generating sequencing-ready Illumina libraries from the resulting enriched DNA fractions. The enrichment protocol utilizes the his-tagged MBD2 protein currently used in the EpiXplore Methylated DNA Enrichment Kit in conjunction with Capturem Nanoprep Columns to separate methylated DNA from unmethylated DNA in ~2 hours. Illumina-compatible sequencing libraries are then generated from the methylated (or unmethylated, if desired) DNA fractions using DNA SMART technology. Categories: Laboratory Molecular & Microbiology Reagents, Nucleic Acid Extraction DNA and RNA Kits. Brand Takara Bio. SKU CLT635023. ID 27375
Medical & Lab Supplies
Guide-it Indel Identification Kit
The Guide-it Indel Identification Kit provides a complete workflow for identifying the variety of insertions and deletions (indels) created using nuclease based genome editing technologies, including CRISPR/Cas9, TALENs, and zinc finger nucleases, in a cell population. The kit contains all of the components needed to amplify, clone, and prepare modified target sites for DNA sequence analysis. The indel identification protocol in this kit has been optimized to provide quick and reliable results. Categories: Laboratory Molecular & Microbiology Reagents, Gene Editing. Brand Takara Bio. SKU CLT631444. ID 24947
Medical & Lab Supplies
Guide-it Mutation Detection Kit
The Guide-it Mutation Detection Kit contains all the reagents needed for PCR-based identification of insertions or deletions generated in the genome of cells treated with engineered nucleases such as zinc-finger nucleases (ZFNs), transcription-activator-like effector nucleases (TALENs), or CRISPR/Cas9. The first step is the amplification of the putative target sequence directly from cells. This kit uses Terra PCR Direct Polymerase Mix and Buffer, so there is no need to extract genomic DNA from your cell population prior to amplification of your target sequence. The amplicon is then melted and hybridized to form the mismatched targets for cleavage by the Guide-it Resolvase. Agarose gel electrophoresis can be used to visualize the cleavage products. Categories: Laboratory Molecular & Microbiology Reagents, Gene Editing. Brand Takara Bio. SKU CLT631443. ID 24931
Medical & Lab Supplies
Heat activated Taq 4x250IU
A highly purified recombinant thermostable DNA Polymerase that has been isolated from E.Coli carrying a vector encoding the Thermus aquaticus DNA polymerase gene. Suitable for PCR-based DNA amplification, cycle sequencing, amplification of cloned inserts. Categories: Laboratory Molecular & Microbiology Reagents, PCR Reagents Polymerases. Brand Fisher Biotec. SKU FBTQTI-3. ID 9253
Medical & Lab Supplies
NucleoSpin 8 / 96 Plasmid
Isolation of plasmid DNA flexible 8-well strip format for varying throughput and proven 96-well plate format for high throughput. Categories: Laboratory Molecular & Microbiology Reagents, Nucleic Acid Extraction Plasmid DNA Kits Molecular Biology Sequencing Grade Plasmid DNA. Brand Macherey-Nagel. SKU MN740621. ID 23825
Medical & Lab Supplies
NucleoSpin 96 Flash
Time and cost efficient high-throughput purification of plasmid DNA. Categories: Laboratory Molecular & Microbiology Reagents, Nucleic Acid Extraction Plasmid DNA Kits Molecular Biology Sequencing Grade Plasmid DNA. Brand Macherey-Nagel. SKU MN740618.2. ID 22397
Medical & Lab Supplies
NucleoSpin 96 Plasmid Transfection Grade
A fast way to purify plasmids for transfection. Categories: Laboratory Molecular & Microbiology Reagents, Nucleic Acid Extraction Plasmid DNA Kits Molecular Biology Sequencing Grade Plasmid DNA. Brand Macherey-Nagel. SKU MN740491.1. ID 28415
Medical & Lab Supplies
NucleoSpin Plasmid EasyPure
For ultra-fast small scale preparation of up to 35 µg of high-purity plasmid DNA. Categories: Laboratory Molecular & Microbiology Reagents, Nucleic Acid Extraction Plasmid DNA Kits Molecular Biology Sequencing Grade Plasmid DNA. Brand Macherey-Nagel. SKU MN740727.10. ID 23919
Medical & Lab Supplies
NucleoSpin Plasmid / Plasmid No Lid
For rapid small scale preparation high-purity plasmid DNA. Categories: Laboratory Molecular & Microbiology Reagents, Nucleic Acid Extraction Plasmid DNA Kits Molecular Biology Sequencing Grade Plasmid DNA. Brand Macherey-Nagel. SKU MN740588.10. ID 23943
Medical & Lab Supplies
NucleoSpin Plasmid Transfection Grade
A fast way to purify plasmids for transfection. Categories: Laboratory Molecular & Microbiology Reagents, Nucleic Acid Extraction Plasmid DNA Kits Molecular Biology Sequencing Grade Plasmid DNA. Brand Macherey-Nagel. SKU MN740490.10. ID 28409
Medical & Lab Supplies
SMARTer® RACE 5'/3' Kit, 10 Rxns
Rapid Amplification of cDNA Ends (RACE) is a technique used to obtain the full-length sequence of an RNA transcript. RACE can provide the sequence of an RNA transcript from a small known sequence within the transcript all the way to the 5' end (5' RACE-PCR) or 3' end (3' RACE-PCR) of the RNA. Categories: Laboratory Molecular & Microbiology Reagents, Nucleic Acid Extraction DNA and RNA Kits. Brand Takara Bio. SKU CLT634858. ID 28451
Medical & Lab Supplies
SMART-Seq® v4 3' DE Kit
Single-cell transcriptomics is important in understanding how gene expression of individual cells varies within cell populations. Takara Clontech has developed an end-capture method that focuses the majority of the sequencing data on the 3' ends of mRNA transcripts from single cells. This enables you to collect high quality data with fewer sequencing reads. This kit incorporates features from the industry-leading SMART-Seq v4 kit, including SMART (Switching Mechanism at 5' End of RNA Template) and locked nucleic acid (LNA) technology. Categories: Laboratory Molecular & Microbiology Reagents, Nucleic Acid Extraction DNA and RNA Kits. Brand Takara Bio. SKU CLT635041. ID 27379
Medical & Lab Supplies
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