Lab Genotype Confirmation Kit NZ Suppliers
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Guide-it Genotype Confirmation Kit Quick enquiry. Where to buy Suppliers range | Every cell has two alleles of most genes. CRISPR/Cas9 gene editing can introduce insertion or deletion mutations (indels) on either one or both alleles of a given gene. The Guide-it Genotype Confirmation Kit provides a simple method to determine if a given clone has mutations in one allele (monoallelic), both alleles (biallelic), or is unchanged (wild type). The protocol involves amplification of the target site from cells treated with Cas9 and a single guide RNA (sgRNA). The resulting amplicon is used in an in vitro cleavage reaction with recombinant Cas9 and the same sgRNA. The genotype can be determined by resolving the cleavage products on an agarose gel. Categories: Laboratory Molecular & Microbiology Reagents, Gene Editing. Brand Takara Bio. SKU CLT632611. ID 27057 | Medical & Lab Supplies |