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Skip sample preparation and go directly to PCR. Terra PCR Direct saves you time and money by allowing you to skip DNA extraction and purification, and go directly to PCR amplification. Terra PCR Direct contains a novel DNA polymerase that lets you amplify directly from animal and plant tissues, as well as from crude DNA extracts. This highly sensitive DNA polymerase also lets you save precious samples, by requiring only a small amount of DNA template. Terra PCR Direct comes preblended with a monoclonal antibody for automatic hot start PCR, lets you easily amplify DNA targets up to 2 kb, and amplifies difficult templates (even when the GC-content is greater than 70%). Categories: Laboratory Molecular & Microbiology Reagents, PCR Reagents. Brand Takara Bio. SKU CLT639286. ID 75306
Medical & Lab Supplies
DNA Polymerase & PCR Kits - Titanium Taq
Titanium Taq DNA Polymerase is a highly sensitive, robust enzyme for use in all PCR applications. It's ideal for the PCR amplification of any DNA template, including bacterial and plasmid DNA, cDNA, and complex genomic DNA. Titanium Taq includes TaqStart Antibody, which provides an integrated hot start for increased specificity and yield. Categories: Laboratory Molecular & Microbiology Reagents, PCR Reagents. Brand Takara Bio. SKU CLT639208. ID 75288
Medical & Lab Supplies
AxyPrep PCR Cleanup Kit (250 preps)
The AxyPrep PCR Cleanup Kit employs a special Binding Solution in combination with an AxyPrep spin column to achieve high selectivity and recovery of DNA fragments. This product is designed to purify DNA fragments > 75 bp from PCRs and other enzymatic reactions, with an expected recovery of 70-90%. It is not necessary to remove mineral oil overlays from the PCRs before purification. This protocol will remove unincorporated primers (<50 not), enzymes and unlabelled and fluorescent dye-labelled mononucleotides. The purified DNA fragments are suitable for a variety of applications, such as sequencing, ligation, restriction analysis, in vitro transcription, etc. Categories: Laboratory Molecular & Microbiology Reagents, Nucleic Acid Extraction Clean Up Kits. Brand Corning Life Sciences. SKU AXY-AP-PCR-250. ID 13564
Medical & Lab Supplies
Easy Yeast Plasmid Isolation Kit, 50 Preps
The Easy Yeast Plasmid Isolation Kit provides a simple and efficient plasmid mini-prep method for rescuing plasmid DNA from yeast (Saccharomyces cerevisiae). The protocol uses Zymolyase for highly efficient enzymatic disruption of yeast cell walls, followed by SDS/alkaline lysis of the resulting spheroplasts. A spin column is used to purify the plasmid DNA, which can then be transformed into E. coli for propagation and scaled-up plasmid preparations, or used as a template for PCR. Categories: Laboratory Molecular & Microbiology Reagents, Nucleic Acid Extraction Plasmid DNA Kits. Brand Takara Bio. SKU CLT630467. ID 15915
Medical & Lab Supplies
In-Fusion® HD Cloning Plus System
In-Fusion PCR Cloning Kits enable directional, seamless cloning of any PCR fragment - or multiple fragments - into any linearized vector in a single 15-minute reaction. No additional treatment of the PCR fragment is required (such as restriction digestion, ligation, phosphorylation, or blunt-end polishing). The efficiency of In-Fusion HD cloning kits is over 95%. Categories: Laboratory Molecular & Microbiology Reagents, Competent Cells and Transfection Reagents. Brand Takara Bio. SKU CLT638909. ID 15933
Medical & Lab Supplies
NucleoCard - Easy extraction of DNA with NucleoSpin
Blood sample storage card for subsequent DNA extraction. Convenient storage of blood samples at room temperature. Suitable for use in real-time PCR assays . Categories: Laboratory Molecular & Microbiology Reagents, Nucleic Acid Extraction Genomic DNA Kits. Brand Macherey-Nagel. SKU MN740403.10. ID 24067
Medical & Lab Supplies
NucleoFast 96 PCR
Cost-efficient 96-well ultrafiltration kit for PCR clean-up. Categories: Laboratory Molecular & Microbiology Reagents, Nucleic Acid Extraction Clean Up Kits. Brand Macherey-Nagel. SKU MN743500.4. ID 24045
Medical & Lab Supplies
NucleoMag 96 PCR
Magnetic-bead based PCR clean-up. Categories: Laboratory Molecular & Microbiology Reagents, Nucleic Acid Extraction Clean Up Kits. Brand Macherey-Nagel. SKU MN744100.1. ID 22717
Medical & Lab Supplies
NucleoSpin 8 / 96 PCR Clean-up
Clean-up of PCR products flexible 8-well strip format for varying throughput and proven 96-well plate format for high throughput. Categories: Laboratory Molecular & Microbiology Reagents, Nucleic Acid Extraction Clean Up Kits. Brand Macherey-Nagel. SKU MN740668. ID 24061
Medical & Lab Supplies
NucleoSpin Gel and PCR Clean-up
PCR clean-up and gel extraction - the two-in-one kit with optimized recovery and elution volume. Categories: Laboratory Molecular & Microbiology Reagents, Nucleic Acid Extraction Clean Up Kits. Brand Macherey-Nagel. SKU MN740609.10. ID 24037
Medical & Lab Supplies
NucleoTraPCR
The silica-matrix based kit for PCR clean-up without columns. Categories: Laboratory Molecular & Microbiology Reagents, Nucleic Acid Extraction Clean Up Kits. Brand Macherey-Nagel. SKU MN740587.10. ID 22411
Medical & Lab Supplies
SMARTer® RACE 5'/3' Kit, 10 Rxns
Rapid Amplification of cDNA Ends (RACE) is a technique used to obtain the full-length sequence of an RNA transcript. RACE can provide the sequence of an RNA transcript from a small known sequence within the transcript all the way to the 5' end (5' RACE-PCR) or 3' end (3' RACE-PCR) of the RNA. Categories: Laboratory Molecular & Microbiology Reagents, Nucleic Acid Extraction DNA and RNA Kits. Brand Takara Bio. SKU CLT634858. ID 28451
Medical & Lab Supplies
SMARTScribe Reverse Transcriptase, 100 Rxns
SMARTScribe Reverse Transcriptase is a high-performance enzyme that allows unbiased cDNA synthesis, mRNA amplification, and library construction from any RNA transcript. SMARTScribe RT is a modified Moloney Murine Leukemia Virus Reverse Transcriptase that generates long, full-length cDNA (up to 14.7 kb) and amplifies rare transcripts, while preserving the relative transcript proportions of the original RNA sample. It has been specially formulated for use with all of our SMART Kits. Categories: Laboratory Molecular & Microbiology Reagents, PCR Reagents. Brand Takara Bio. SKU CLT639537. ID 15911
Medical & Lab Supplies
Universal Genome Walker 2.0
The Universal Genome Walker 2.0 Kit is a complete kit that contains all the reagents you need to construct gene libraries and perform PCR-based walking" in the genomic DNA of any species. In addition to the Universal Genome Walker library construction components, the kit includes an Advantage 2 PCR Kit as well as the NucleoSpin Tissue and NucleoSpin Gel and PCR Clean-Up Kits. The Genome Walker Universal 2.0 Kit supplies sufficient reagent for the generation of three gene libraries from three different samples and the performance of 80 walks. All you need to add are your own gene-specific primers (GSP). Categories: Laboratory Molecular & Microbiology Reagents, Nucleic Acid Extraction Genomic DNA Kits. Brand Takara Bio. SKU CLT636405. ID 15919
Advantage GC 2 Polymerase Mix is an excellent choice for GC rich PCR applications. For example, GC-rich templates, such as the 5' ends of many mammalian cDNAs, cannot be amplified using conventional PCR techniques. Advantage GC 2 includes Advantage 2 Polymerase Mix, our proprietary GC-Melt Reagent and a reaction buffer containing DMSO - two proven additives allowing amplification of sequences that are up to 90% GC with minimal buffer optimization. Categories: Laboratory Molecular & Microbiology Reagents, PCR Reagents. Brand Takara Bio. SKU CLT639119. ID 75302
Medical & Lab Supplies
Guide-it Mutation Detection Kit
The Guide-it Mutation Detection Kit contains all the reagents needed for PCR-based identification of insertions or deletions generated in the genome of cells treated with engineered nucleases such as zinc-finger nucleases (ZFNs), transcription-activator-like effector nucleases (TALENs), or CRISPR/Cas9. The first step is the amplification of the putative target sequence directly from cells. This kit uses Terra PCR Direct Polymerase Mix and Buffer, so there is no need to extract genomic DNA from your cell population prior to amplification of your target sequence. The amplicon is then melted and hybridized to form the mismatched targets for cleavage by the Guide-it Resolvase. Agarose gel electrophoresis can be used to visualize the cleavage products. Categories: Laboratory Molecular & Microbiology Reagents, Gene Editing. Brand Takara Bio. SKU CLT631443. ID 24931
Medical & Lab Supplies
Premix Ex Taq (Perfect Real Time)
Premix Ex Taq (Perfect Real Time) is a 2X RT-PCR kit specifically designed for fast and sensitive real-time PCR via either SYBR Green Real-Time PCR (qPCR) or probe-based qPCR assays. SYBR Green I Dye and probes for probe/5' nuclease based assays are not included in this RT-PCR kit. The Premix Ex Taq (Perfect RealTime) kit consists of Takara's high fidelity and high performance TaKaRa Ex Taq Hot Start polymerase and a real-time buffer that ensures superior specificity and increased amplification efficiency during RT-PCR (qPCR). Antibody-mediated hot start technology prevents nonspecific amplification due to mispriming and/or formation of primer-dimers during room temperature reaction assembly. Once the Taq antibody-polymerase complex is denatured during the first cycling step, the Premix Ex Taq polymerase can initiate DNA synthesis. Categories: Laboratory Molecular & Microbiology Reagents, PCR Reagents. Brand Takara Bio. SKU TKRR039A. ID 74850
Medical & Lab Supplies
Real-time RT-PCR Kit: PrimeScript RT Master Mix (Perfect Real Time)
To minimize sample handling time, look no further than this real-time RT-PCR kit. PrimeScript RT Master Mix (Perfect Real Time) is designed for fast, efficient reverse transcription prior to real-time RT-PCR (qPCR) with a streamlined workflow. cDNA synthesis reactions are easy to assemble - just add RNA template and water. The real-time RT-PCR kit contains a 5X master mix reagent that includes all reagents required for cDNA synthesis (PrimeScript RTase, RNase Inhibitor, random 6mers, Oligo dT Primer, dNTP Mixture, and reaction buffer). Because this real-time RT-PCR kit is powered by PrimeScript Reverse Transcriptase, it enables full-length cDNA synthesis for real-time RT-PCR (qPCR) in just 15 minutes. Categories: Laboratory Molecular & Microbiology Reagents, PCR Reagents. Brand Takara Bio. SKU TKRR036A. ID 35222
Medical & Lab Supplies
Simplexa BKV Kit 100 Tests
A real-time PCR assay intended for the in vitro quantitative detection of BK Virus. Categories: Laboratory Molecular & Microbiology Reagents, Nucleic Acid Extraction. Brand Focus Diagnostics. SKU FD-MOL2300. ID 24509
Medical & Lab Supplies
Simplexa Bordetella Kit 100 Tests
A real-time PCR assay for the in vitro qualitative direct detection and discrimination of Bordetella pertussis and/or Bordetella parapertussis DNA. Extraction and amplification is performed in one protocol with no separate nucleic acid extraction step. This kit contains sufficient reagents to perform 100 reactions directly from the specimen. Categories: Laboratory Molecular & Microbiology Reagents, Elisa and Immunoassays. Brand Focus Diagnostics. SKU FD-MOL2700. ID 24503
Medical & Lab Supplies
Simplexa C. difficle Universal Direct Kit 100 tests
A real-time PCR assay for the in vitro qualitative direct detection of the toxin B gene (tcdB) of C. difficile. Extraction and amplification is performed in one protocol with no separate nucleic acid extraction step. Categories: Laboratory Molecular & Microbiology Reagents, Elisa and Immunoassays. Brand Focus Diagnostics. SKU FD-MOL2975. ID 24501
Medical & Lab Supplies
Simplexa EBV Kit 100 Tests
A real-time PCR assay intended for the in vitro quantitative detection of Epstein-Barr virus. Categories: Laboratory Molecular & Microbiology Reagents, PCR Reagents. Brand Focus Diagnostics. SKU FD-MOL2400. ID 24505
Medical & Lab Supplies
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